miR-19a-3p inhibition alleviates sepsis-induced lung injury via enhancing USP13 expression

Sepsis is a systemic inflammatory response syndrome caused by various pathogenic microorganisms or toxins. Lung damage one of the causes death in patients with sepsis. This study aimed to investigate role miR-19a-3p and its regulation mechanism sepsis-induced lung injury. MH-S cells were treated lipopolysaccharide (LPS) establish injury cell model. C57BL/6 mice injected antagomiR LPS construct animal LPS-treated control transfected mimic, inhibitor USP13 expression vector . The levels examined quantitative real-time polymerase chain reaction (qRT-PCR) Western blotting. concentration cytokines was measured enzyme-linked immunosorbent assay (ELISA). relationship validated using dual-luciferase reporter assay. assessed hematoxylin-eosin staining (HE). results showed that treatment increased TNF-?, IL-6 IL-1? cells. In cells, level gradually over time. Both knockdown overexpression inhibited LPS-induced production IL-1?. Moreover, negatively regulated Furthermore, suppressed sepsis model mice. conclusion, could alleviate through enhancing expression.